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Plasmid#185487PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTALE64G
Plasmid#35188PurposeGolden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP additionDepositorInsertTAL Effector-VP64-T2A-eGFP
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 Domain, T2A-GFPPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG30
Plasmid#220740PurposeNIR-FbLAG30 expression in mammalian cells.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor
Plasmid#25938DepositorInsertClopHensor
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PalmPalm-ClopHensor
Plasmid#25940DepositorInsertPalmPalm-ClopHensor
UseTagsGap20 and Strep-IIExpressionMammalianMutationN-term (20AA) of Gap-43PromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-gap16-ClopHensor
Plasmid#26006DepositorInsertgap16-ClopHensor
UseTagsGap16 and Strep-IIExpressionMammalianMutationN-term (16AA) of Gap-43PromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
RG6
Plasmid#80167Purposefluorescent protein splicing reporterDepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NPY-ClopHensor
Plasmid#25939DepositorInsertNPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
UseTagsNPY and Strep-IIExpressionMammalianMutationNeuropeptide Y localization signalPromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
FRE5Cf
Plasmid#62378PurposeDerived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.DepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry
Plasmid#124493PurposeExpresses FlipYFP (TEV cleavage sequence) and T2A mCherryDepositorInsertFlipYFP(TEV cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PA-Bongwoori-R3
Plasmid#137838PurposeA photoactivatable GEPI variant originated from ecliptic pHluorinDepositorInsertPhotoactivatable_Bongwoori-R3
UseTagsExpressionMammalianMutationL64F, T65S, V163A, T203H, A227DPromoterCMVAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY094
Plasmid#84743PurposeExpresses huAsCpf1-T2A-GFP and crRNA guideDepositorInsertshuAsCpf1
GFP
UseTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY095
Plasmid#84744PurposeExpresses huLbCpf1-T2A-GFP and crRNA guideDepositorInsertshuLbCpf1
GFP
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDXC3GMS
Plasmid#49031PurposeFX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptideDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), EGFP, MYC, a…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSMG3
Plasmid#49029PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), EGFP, MYC, a…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only