Addgene

We narrowed to 13,814 results for: pET

Showing: 541 - 560 of 13814 results

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pET-30 Ubwt (2)

Plasmid

#26685

Depositor

Linda Hicke

Article

French et al J Biol Chem. 2009 May 1. 284(18):12071-9

Insert

Ubiquitin

Use

Tags

His and S-tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-IL-2 signal_CyPet

Plasmid

#128029

Purpose

Mammalian expression vector with N terminal IL-2 signal peptide and CyPet

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

CyPet and IL-2 signal peptide

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET-X(5010)B1

Plasmid

#168019

Purpose

Expresses X(5010)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(5010)B1-6xHis

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR

Plasmid

#119828

Purpose

Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D2

Plasmid

#15783

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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nifF in pET21b

Plasmid

#124520

Purpose

Azotobacter vinelandii flavodoxin II of Nitrogenase

Depositor

Douglas Rees

Article

Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

Insert

Flavodoxin II (nifF Azotobacter vinelandii)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

T7 / lac

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Article

Chivers et al Nat Methods. 2010 Apr 11. ():.

Insert

Traptavidin

Use

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pET-CV-Ykt6

Plasmid

#168030

Purpose

Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Ykt6(Q131-K190)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-CV-SNAP25

Plasmid

#168031

Purpose

Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-SNAP25(A141-G206)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Promoter

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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