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Plasmid#199363PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pHR-flag-Lag16-iCAM-1
Plasmid#205195PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP
Plasmid#199359PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSlmb-vhhGFP4
Plasmid#35579DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-Ecad
Plasmid#205197PurposeThis vector encodes of the synCAM (Ecad) and GFP nanobody LAG16DepositorInsertsynCAM Ecad and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP CMV VHHG4
Plasmid#182236PurposeExpresses GFP nanobody for stable expression in mammalian cellsDepositorInsertanti-GFP nanobody
UseLentiviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS
Plasmid#199348PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-TEVExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP
Plasmid#199360PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(294) pcDNA3.1-myc-OGA(1-400)
Plasmid#168095PurposeMyc tagged truncated human OGA catalytic domain of residues 1-400DepositorInsertmyc-OGA(1-400)
UseTagsmycExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-JAM-B
Plasmid#205200PurposeThis vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16DepositorInsertsynCAM JAM-B and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RBD-JA1
Plasmid#167126PurposeExpresses SARS-CoV-2 RBD in bacterial systemDepositorInsertSARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
UseTagsExpressionBacterialMutationresidues (333 to 529 aa)PromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAST_NSlmb-vhhGFP4
Plasmid#35575DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP
Plasmid#199358PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
hP2X3-cALFA
Plasmid#160499PurposeFor the expression of human P2X3 with ALFA tag at C-terminus in mammalian cellsDepositorInserthuman P2X3 (P2RX3 Synthetic, Human)
UseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationΔN5ΔC33-T13P/S15V/V16IPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Gal4-GBP2-IRES-GBP7-p65
Plasmid#50020PurposeMulti-purpose expression of a GFP-dependent transcription factorDepositorInsertGal4DBD-GBP2-IRES-NLS-GBP7-p65AD
UseMulti-purpose expressionTagsExpressionMutationPromoterCMV-immediate early promoter and SP6 promoterAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP1-10gly-Gal4DBD
Plasmid#49438PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP1-Gal4 DNA binding domain fusion protein
UseTagsExpressionMammalianMutationC98S (to increase protein solubility), Q3DPromoterCAGAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS
Plasmid#199347PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only