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pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

Plasmid

#80860

Purpose

Phagemid (encodes evolved cI5C6A,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pZA2[PTAN:GFP]

Plasmid

#60779

Purpose

Contains PTAN driving expression of GFP

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

yemGFP

Use

Tags

Expression

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZA2[PLac:mCherry,PTAN:GFP]

Plasmid

#60780

Purpose

Contains PLac driving expression of mCherry and PTAN driving expression of GFP.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

mCherry
yemGFP

Use

Tags

Expression

Mutation

Promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJB066

Plasmid

#98913

Purpose

Express FtsZ tagged with pAmCherry1 in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Use

Tags

pAmCherry1

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR puroR R4-R3

Plasmid

#43965

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLO104

Plasmid

#90224

Purpose

Complements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_00531

Use

Aspergillus

Tags

Expression

Mutation

Promoter

pLac

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pF-01

Plasmid

#166931

Purpose

GFP coding region part (F)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Green Fluorescent Protein - Block F- Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pD-01

Plasmid

#166929

Purpose

TrrnB and L3S2P21 terminator part (D)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

DOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLO99

Plasmid

#90219

Purpose

Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_03691

Use

Aspergillus

Tags

Expression

Mutation

Promoter

pLac

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO101

Plasmid

#90221

Purpose

Complements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_07102

Use

Aspergillus

Tags

Expression

Mutation

Promoter

pLac

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO102

Plasmid

#90222

Purpose

Complements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_02347

Use

Aspergillus

Tags

Expression

Mutation

Promoter

pLac

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-36

Plasmid

#63825

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

Plasmid

#80864

Purpose

Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5C6G7G,P

Use

Tags

Expression

Bacterial

Mutation

238G to 238D (please see depositor comment below)

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRoDonor

Plasmid

#178773

Purpose

Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII

Plasmid

#80862

Purpose

Phagemid (encodes evolved cI5G6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-IRES-puro-polyA

Plasmid

#43967

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Use

Gateway dest vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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