-
Plasmid#73440PurposeViolacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.DepositorInsertPG6-vioABE-P4A6-vioC-P3A2-vioD
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6, P4A6, and P3A2 (orthogonal T7-lac variants)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mEmerald-CAPRIN1
Plasmid#164218PurposeExpresses mEmerald tagged CAPRIN1 under EF-1a promoterDepositorInsertCAPRIN1 (CAPRIN1 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF-1aAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-LAMP1-HaloTag
Plasmid#164216PurposepLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsHaloTagExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-LAMP1-HaloTag
Plasmid#164209PurposeExpresses HaloTag tagged LAMP1 under CMV promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-PGK-LAMP1-EYFP
Plasmid#164215PurposepLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsEYFPExpressionMutationPromoterPGKAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-ANXA11-mCerulean
Plasmid#164214PurposepLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmCeruleanExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)
Viral Prep#112677-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)
Viral Prep#112677-AAV1PurposeReady-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)
Viral Prep#112677-AAV8PurposeReady-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)
Viral Prep#112677-AAV9PurposeReady-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)
Viral Prep#112677-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11
Plasmid#164203PurposeExpresses CRY2oligo-mcherry tagged ANXA11 under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MLRV2: NF-kb-SMAD-DBE-P53-AP1
Plasmid#124536PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1DepositorInserts5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald D40G
Plasmid#164211PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationD40GPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(D40G)
Plasmid#164204PurposeExpresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationD40GPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)
Plasmid#164208PurposeExpresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutation186-505 aaPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(1-185aa)
Plasmid#164207PurposeExpresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutation1-185 aaPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R235Q)
Plasmid#164205PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationR235QPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R346C)
Plasmid#164206PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationR346CPromoterCMVAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only