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Plasmid#231617PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS2
Plasmid#231616PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS5
Plasmid#231614PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPbad-TetR-sfGFP-Ptet-AraC-mKate2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS4
Plasmid#231613PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPtet-AraC-mKate2-Pbad-TetR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11
Plasmid#154025PurposeBichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7
Plasmid#154023PurposeBichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.5t
Plasmid#210257PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertseBFP2
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.1t
Plasmid#210254PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCerulean
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.6t
Plasmid#210258PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmScarlet
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7-D192D
Plasmid#154024PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.576C>T, p.D192DPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11-G312G
Plasmid#154026PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.936T>G, p.G312GPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A
Plasmid#159170PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A
UseTagsExpressionMammalianMutationMutated Met 7 of the cyt b562 module of hHS1 to A…PromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRR5
Plasmid#225095Purposeparental riboswitch reporter vector for regulation of the gfpUV fluorescent proteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotersyntheticAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMini-CAAGs::RFP-DR48
Plasmid#132640PurposeFluorescent reporter to quantify elicitation of the strand annealing mediated repair pathwayDepositorInsertmRFP
UseTagsExpressionMammalianMutationContains a 92bp insertion interrupting the open …PromoterChicken B-actinAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A,H102A
Plasmid#159171PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A,H102A
UseTagsExpressionMammalianMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
UseTagsLckExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only