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Plasmid#127710PurposeLevel 0 destination vector for a promoter with A - B scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCas2B
Plasmid#82373PurposedCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas8
Plasmid#82380PurposedCas9 without a promoter in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas7
Plasmid#82379PurposedCas9 expressed from low constituve promoter, c225, in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoterc225Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2A
Plasmid#82372PurposedCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas28
Plasmid#82385PurposesgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23108Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas27
Plasmid#82384PurposesgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23117Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMY07AB-ORF
Plasmid#127711PurposeLevel 0 destination vector for a coding sequence with B - C scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY07CD-TER
Plasmid#127712PurposeLevel 0 destination vector for a terminator with C - D scars. Clone in with BpiI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a
Plasmid#127716PurposeLevel 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ab
Plasmid#127717PurposeLevel 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc
Plasmid#127718PurposeLevel 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd
Plasmid#127719PurposeLevel 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de
Plasmid#127720PurposeLevel 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef
Plasmid#127721PurposeLevel 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3
Plasmid#127722PurposeLevel 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
J72008 bacterial strain with plasmid
Bacterial Strain#26187DepositorBacterial ResistanceAmpicillinAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only