Purpose
SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
Inserts
humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
Use
CRISPR and Mouse Targeting
Tags
3xFLAG and T2A-PuroR
Expression
Mammalian
Mutation
D10A mutant converts to Nickase
Available since
Dec. 14, 2017
Availability
Academic Institutions and Nonprofits only