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Plasmid#191334PurposeExpresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-KIFC1 (KIFC1 Human)
UseTagsExpressionMammalianMutationmotor domain aa 125-673; N593KPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T
Plasmid#191331PurposeExpresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-UbC-3xNLS-mScarlet-I
Plasmid#191099PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet-I
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationoptimized to human codon usagePromoterUbCAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PARL-D319N-FLAG C-t
Plasmid#191957PurposeExpression of Presenilin Associated Rhomboid Like (PARL) D319N mutantDepositorInsertPARL D319N (PARL Human)
UseTagsFLAGExpressionMammalianMutationD319NPromoterAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
UseTags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
UseTagsmScarlet fused to FKBPExpressionYeastMutationPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-TsCC(B)::KANMX
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
UseTagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastMutationPromoternoneAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)
Plasmid#181861PurposeFluoSTEP-AKAR using mScarlet-I as the FRET acceptor.DepositorInsertFluoSTEP-AKAR (mScarlet-I)
UseTagsmScarlet-I and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-gB-BlastR [M1G]
Plasmid#171816PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.DepositorInsertmScarlet-F-gB-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-gB-PuroR [M1G]
Plasmid#171812PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1DepositorInsertmScarlet-F-gB-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA847 - mec-4p::hrp-1mScarlet
Plasmid#139201PurposeExpresses mScarlet tagged hrp-1 in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA848 - mec-4p::hrp-1HsLCWTmScarlet
Plasmid#139202PurposeExpresses mScarlet tagged hrp-1HsLCWT in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA849 - mec-4p::hrp-1HsLCD290VmScarlet
Plasmid#139203PurposeExpresses mScarlet tagged hrp-1HsLCD290V in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA844 - hrp-1p::hrp-1mScarlet
Plasmid#139204PurposeExpresses mScarlet tagged hrp-1DepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA845 - hrp-1p::hrp-1HsLCWTmScarlet
Plasmid#139205PurposeExpresses mScarlet tagged hrp-1HsLCWTDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet
Plasmid#139206PurposeExpresses mScarlet tagged hrp-1HsLCD290VDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-C1-WPRE
Plasmid#159955Purposeuntargeted markerDepositorInsertmScarlet-C1
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Scarlet-Borcs7-Q87X
Plasmid#118751PurposeExpresses scarlet tagged Borcs7-Q87XDepositorInsertBorcs7-Q87X cDNA (2010012O05Rik Mouse)
UseTagsmScarletExpressionMammalianMutationQ87XPromoterCAGAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL1-V5-His6
Plasmid#67416PurposeExpression of hArl1 with C-term V5 and His6 tag in E. coliDepositorInsertARL1 (ARL1 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 231 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL2-V5-His6
Plasmid#67417PurposeExpression of hArl2 with C-term V5 and His6 tag in E. coliDepositorInsertARL2 (ARL2 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only