Addgene

We narrowed to 961 results for: aip

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Actin-mRFP-PAGFP

Plasmid

#62382

Purpose

Expresses beta actin with both a PAGFP and mRFP tag.

Depositor

Tim Mitchison, Guillaume Charras

Article

Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

Insert

beta actin (ACTB Human)

Use

Tags

PAGFP and mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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VN-Sph1

Plasmid

#89755

Purpose

expresses human Synphilin fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

Insert

SNCAIP (SNCAIP Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Use

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-PRRX1

Plasmid

#215024

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

paired related homeobox 1 (PRRX1 Human)

Use

AAV

Tags

FKBP-V5

Expression

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_P139S

Plasmid

#215040

Purpose

Mammalian expression of human TWIST1 (with P139S mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

P139S

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_L138Y

Plasmid

#215041

Purpose

Mammalian expression of human TWIST1 (with L138Y mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

L138Y

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_L138F

Plasmid

#215042

Purpose

Mammalian expression of human TWIST1 (with L138F mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

L138F

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_MSX1-FKBP-mNeonGreen-V5

Plasmid

#215025

Purpose

AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

msh homeobox 1 (MSX1 Human)

Use

AAV

Tags

FKBP-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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psicheck2-PHLPP2mut

Plasmid

#203606

Purpose

Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR were m…

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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CSII-TK-B4GALT3

Plasmid

#203598

Purpose

Lentiviral vector to express B4GALT3 in mammalian cells

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

WT

Promoter

TK

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3

Plasmid

#203601

Purpose

Reporter plasmid carrying B4GALT3 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

nt 1800-2330 3'UTR

Promoter

SV40

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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CARPID dCasRx-BASU

Plasmid

#153303

Purpose

express CARPID dCasRx-BASU fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

dCasRx-BASU

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ALX4-Flag

Plasmid

#215029

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-TWIST1

Plasmid

#215022

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

AAV

Tags

FKBP-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_ALX4-Flag-HA

Plasmid

#215028

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Use

Tags

Flag-HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF692

Plasmid

#88701

Purpose

Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF692 (ZNF692 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-ORF9B

Plasmid

#154425

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9B

Use

Gateway-compatible entry vector, with insert of o…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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