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Plasmid#34627DepositorInsertEF-Sep (tufB E. coli)
UseTags6xHIS and MBPExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-YFP-NLS
Plasmid#145274PurposeMammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLSDepositorInsertMCS and YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST2-sfGFP
Plasmid#126575PurposeStrong constitutive expression of super-folder GFP for cell-free expressionDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_PTS2
Plasmid#65676PurposeExpresses PTS2-CNL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI3up
Plasmid#176170Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3DepositorInsert5' homology for integration site XI-3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI3dw
Plasmid#176171Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3DepositorInsert3' homology for integration site XI-3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI5up
Plasmid#176172Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5DepositorInsert5' homology for integration site XI-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI5dw
Plasmid#176173Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5DepositorInsert3' homology for integration site XI-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XII5up
Plasmid#176176Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5DepositorInsert5' homology for integration site XII-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XII5dw
Plasmid#176177Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5DepositorInsert3' homology for integration site XII-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-X2up
Plasmid#176162Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2DepositorInsert5' homology for integration site X-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-X2dw
Plasmid#176163Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2DepositorInsert3' homology for integration site X-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEX-GECO1
Plasmid#32464DepositorArticleInsertGEX-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 464
Plasmid#138068PurposeNatural Terminator ECK120033736 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-33736-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only