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Showing: 541 - 560 of 2455 results

  1. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

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  2. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  4. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  5. AA516

    Plasmid
    #231118
    Purpose
    Fragmid fragment: (2A Selection) CBE activity-based selection intron
    Depositor
    David Root, John Doench
    Insert
    PuroN'-intron-PuroC'
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231118-plasmid-map-sequence-id-458371

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  6. AA509

    Plasmid
    #231116
    Purpose
    Fragmid fragment: (2A Selection) ABE activity-based selection intron
    Depositor
    David Root, John Doench
    Insert
    PuroN'-intron-PuroC'
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231116-plasmid-map-sequence-id-458354

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  7. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  10. pJK520

    Plasmid
    #72479
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a and none
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72479-plasmid-map-sequence-id-132532

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  11. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

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  12. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

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  13. pJK604

    Plasmid
    #71610
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71610-plasmid-map-sequence-id-131686

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  14. pJK664

    Plasmid
    #71613
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71613-plasmid-map-sequence-id-131691

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  15. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  16. pJK563

    Plasmid
    #71689
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71689-plasmid-map-sequence-id-131681

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  17. pJK508

    Plasmid
    #71598
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71598-plasmid-map-sequence-id-131672

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  18. pCVD529

    Plasmid
    #66159
    Purpose
    A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322
    Depositor
    James Kaper
    Article
    Nishibuchi et al Mol Microbiol. 1990 Jan;4(1):87-99.
    Insert
    tdh2
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 541 - 560 of 2455 results