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Plasmid#202556PurposeSynaptotagmin-1 sgRNA3 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)
Plasmid#202553PurposeNontargeting control vector with TagBFP2DepositorInsertHumanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPuromycin-resistance gene in the pLV hU6-sgRNA hU…PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)
Plasmid#202554PurposeSynaptotagmin-1 sgRNA1 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
Plasmid#159557PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationG13DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159552PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationQ61RPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159559PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationQ61RPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X29-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V
Plasmid#159549PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG12VPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159555PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationG12DPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
Plasmid#159558PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationQ61LPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide4
Plasmid#125411PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide4
Plasmid#125451PurposeCRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide3
Plasmid#125450PurposeCRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide2
Plasmid#125449PurposeCRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide1
Plasmid#125448PurposeCRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide4
Plasmid#125443PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide3
Plasmid#125442PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide2
Plasmid#125441PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide4
Plasmid#125435PurposeCRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide3
Plasmid#125434PurposeCRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide2
Plasmid#125433PurposeCRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only