Addgene

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046426

Plasmid

#149140

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7b

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_16.10

Plasmid

#178914

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBEL1810

Plasmid

#139914

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L358N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046555

Plasmid

#149146

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAJ12

Plasmid

#14914

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMAL358

Plasmid

#169147

Purpose

Encodes OptoQ-AMP1

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-TCF4

Plasmid

#109144

Purpose

For the expression of GFP-TCF in mammalian cells

Depositor

Mariann Bienz

Article

Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of BFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAV-5S-Squash

Plasmid

#177914

Purpose

Expresses 5S rRNA fused to Squash aptamer in mammalian cells

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Squash aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

5S

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt7A

Plasmid

#35914

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFlpStop-HDR-UAS-2.1-tdTom

Plasmid

#89148

Purpose

FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

5XUAS, 3XP3-dsRed

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMulti-sgRNA-LacZ-DsRed

Plasmid

#99914

Purpose

Receptor plasmid for the assembly of multiple sgRNAs with DsRed coexpression

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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