Addgene

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pdCas9- AsiA_m1.1

Plasmid

#158064

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 1.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pZA27

Plasmid

#158505

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Use

Tags

4xMyc

Expression

Plant

Mutation

L17E

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pSI-356 v2

Plasmid

#158431

Purpose

SpCas9 sgRNA cloning backbone

Depositor

Nozomu Yachie

Article

Ishiguro et al Methods Mol Biol. 2021;2312:193-223. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human H6

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMOR21-1-ISH

Plasmid

#15838

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR21-1 (Olfr571 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pAAV_2pabPAC

Plasmid

#158969

Purpose

2P-activatable bPAC for AAV

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Sticky-147-4X

Plasmid

#158575

Purpose

Production of DNA fragment for nucleosome core particle preparation

Depositor

Curt Davey

Article

Sharma et al Nat Commun. 2019 Dec 17;10(1):5751. doi:

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS4-blast

Plasmid

#158473

Purpose

Constitutive lentiviral expression of human TMPRSS4.

Depositor

Sho Iketani, Alejandro Chavez

Insert

TMPRSS4 (TMPRSS4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ICK_IDG-K

Plasmid

#158187

Purpose

Gateway destination clone of ICK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-ICK (CILK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Use

Tags

3xFlag and BirA*

Expression

Mammalian

Mutation

Promoter

miniCMV

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-bovine-tau

Plasmid

#15864

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

bovine tau (MAPT )

Use

Tags

Expression

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pMOR103-9-ISH

Plasmid

#15850

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR103-9 (Olfr1348 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pO-macs-ISH

Plasmid

#15856

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Olfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pMOR10-ISH

Plasmid

#15857

Depositor

Peter Mombaerts

Article

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

MOR10 (Or4e1 Mouse)

Use

For riboprobe, in situ hybridization

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pMOR22-2-ISH

Plasmid

#15839

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR22-2 (Olfr69 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pMOR31-2-ISH

Plasmid

#15844

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR31-2 (Olfr690 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pISH-V2ra

Plasmid

#15877

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

V2ra

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only