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Showing: 1081 - 1120 of 158707 results

  1. pAAVS1-shRNA

    Plasmid
    #82697
    Purpose
    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.
    Depositor
    Alan Mullen
    Article
    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    hPGK, U6
    Available since
    Nov. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    82697-plasmid-map-sequence-id-157348

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  3. pOpen-KTHEAQpol

    Plasmid
    #165542
    Purpose
    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    KlenTaq1 polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165542-plasmid-map-sequence-id-339275

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  6. MC1R-Tango

    Plasmid
    #66427
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC1R (MC1R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  18. MC2R-Tango

    Plasmid
    #66428
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC2R (MC2R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  23. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  25. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  28. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    hmpAp
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  36. Anti-Pan-Neurofascin (extracellular) [A12/18R]

    Plasmid
    #177438
    Purpose
    Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177438-plasmid-map-sequence-id-348771

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  38. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

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  39. meGFP-hTRAK1

    Plasmid
    #188664
    Purpose
    human TRAK1 with N-terminal meGFP tag
    Depositor
    Thomas Schwarz
    Insert
    OIP106 (TRAK1 Human)
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188664-plasmid-map-sequence-id-369503

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  40. pEPQD0KN0750

    Plasmid
    #185627
    Purpose
    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    lacZ
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185627-plasmid-map-sequence-id-368554

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Showing: 1081 - 1120 of 158707 results