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Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-EGFP-IRES2-mCherry (FACS reporter)
Plasmid#222999PurposeFACS reporter constitutively expressing mCherry and conditionally expressing EGFP upon prime editing. For lentivirus packaging.DepositorInsertEGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inse…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-RIPK1
Plasmid#78846PurposeTo detect localization of RIPK1 protein in live mammalian cellsDepositorInsertRIPK1 (RIPK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIKOrbsARCm
Plasmid#46765PurposeE.coli genomic integration rbsAR locusDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE (AAV Retrograde)
Viral Prep#176752-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV9)
Viral Prep#100848-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62
Plasmid#204548PurposeExpresses p62 in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-CCR5_DF_B584b_attB
Plasmid#182148PurposeTo insert attB attachment site at CCR5 in human cells via twinPEDepositorInsertCCR5-B584b-attB pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-CCR5_DF_A509b_attB
Plasmid#182147PurposeTo insert attB attachment site at CCR5 in human cells via twinPEDepositorInsertCCR5-A509b-attB pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A38
Plasmid#132053PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A38 (SLC25A38 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits