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Plasmid#104608PurposeExpresses TALE4 under UAS controlDepositorInsertTALE4
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA
Plasmid#87587PurposePV8 for 3-input, 2-output Full AdderDepositorInsertEGFP, mCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AU1-TTC30A2-pcDNA3
Plasmid#47324PurposeExpresses AU1 epitope-tagged TTC30A2 in mammalian cellsDepositorInsertTTC30A2 (Ttc30a2 Mouse)
UseTagsAU1 epitopeExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 L5-L4
Plasmid#62102PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV220: CoChR(C108S)-eGFP in fck
Plasmid#58512PurposeExpresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoterDepositorInsertCoChR(C108S)-eGFP
UseLentiviralTagseGFPExpressionMammalianMutationchanged Cysteine 108 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-Ui4-eSIBR
Plasmid#82586Purpose5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAiDepositorInserthuman ubiquitin C promoter followed by intronically expressed eSIBR cassette
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 L1-L4
Plasmid#62112PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR
Plasmid#61778PurposeLuciferase reporter containing the 3'UTR of mouse YwhagDepositorInsertmouse Ywhag 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 LxCxE HA
Plasmid#8957DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationDeletion of LxCxE motifPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_3_TOPO_TA-ms2-VP64
Plasmid#79371PurposeRecruits VP64 to a compatible gRNA for transcriptional activationDepositorInsertMS2-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3A
Plasmid#79501PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDSDepositorInsertWNT3A (WNT3A Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA M4
Plasmid#80937PurposeExpresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct:MCP-VP64
Plasmid#78904PurposeExpresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertMS2:VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceMarch 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR
Plasmid#61779PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1DepositorInsertmouse Xbp1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[GFPApt]
Plasmid#68428PurposeTransient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the GFP aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xK-T]
Plasmid#68429PurposeTransient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three kink-turns
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only