Addgene

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pTU2-A-RFP

Plasmid

#72954

Purpose

Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - A site

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3

Plasmid

#79500

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3 (WNT3 Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Tags

Expression

Worm

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCS1

Plasmid

#55749

Purpose

Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-R5

Plasmid

#62111

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBGW

Plasmid

#62665

Purpose

Donor vector for mCitrine knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

mCitrine

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGX

Plasmid

#62666

Purpose

Donor vector for Dre knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

Dre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBEZ

Plasmid

#62669

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBFB

Plasmid

#62668

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30B-pcDNA3

Plasmid

#47325

Purpose

Expresses AU1 epitope-tagged TTC30B in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30B (Ttc30b Mouse)

Use

Tags

AU1 epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor mCherry-PA

Plasmid

#72596

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

mCherry-puromycin cassetter

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

Plasmid

#104614

Purpose

Expresses GFP upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS3

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

Plasmid

#104613

Purpose

Expresses GFP upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS1

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE1-VP64-P10

Plasmid

#104606

Purpose

Expresses TALE1 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE1

Use

TALEN

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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