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Plasmid#64114PurposeVector for expression of Sp sgRNA2 in mammalian cellsDepositorInsertSp sgRNA2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCS104
Plasmid#209357PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-cPLA2-mEGFP
Plasmid#184718PurposeExogenous cPLA2WTDepositorInsertcPLA2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX - T7pro - EBFP
Plasmid#138615PurposeExpress EBFP with an upstream T7 promoter in a lentiviral backboneDepositorInsertEBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRA99
Plasmid#209334PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEiLOV-N1
Plasmid#26588DepositorInsertiLOV
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-LOV*-H2B
Plasmid#202204PurposeExpresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cellsDepositorInsertHistone H2B type 1-K
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W
Plasmid#112925PurposeLentiviral vector for SAM-CRISPRa gRNA expression with puroBFPDepositorInserthU6 promoter, gRNA-SAM expression cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19 - T7 pro - IRES - EGFP
Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nmdCas9-3XmCherry
Plasmid#64110PurposeExpression of Nm dCas9-3XmCherry in mammalian cellsDepositorInsertNm dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2(1-10)
Plasmid#82603PurposeLentiviral vector for constitutive expression of sfCherry2(1-10)DepositorInsertsfCherry2(1-10)
UseLentiviralTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterSFFVAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH-nmsgRNA1.1
Plasmid#64115PurposeVector for expression of Nm sgRNA1.1 in mammalian cellsDepositorInsertNm sgRNA1.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG469-LifeAct-redFAST
Plasmid#160365PurposeExpresses LifeAct-redFAST in mammalian cellsDepositorInsertLifeAct-RedFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFCcGi
Plasmid#59324PurposeInducible expression of GFP and constitutive expression of mCherryDepositorInsertsmCherry
EGFP
UseTagsExpressionBacterialMutationPromoterPbad and rpsMAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only