Addgene

We narrowed to 6,298 results for: sms

Showing: 5501 - 5520 of 6298 results

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px330 RBP1 gRNA

Plasmid

#165593

Purpose

Insertion of RBP1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

RBP1 (Polr2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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NET39 pEGFP-C1 (1045)

Plasmid

#62024

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pEF-pIgH-ESCargo*

Plasmid

#140159

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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miniSOG-Lifeact-7

Plasmid

#54823

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Use

Tags

Lifeact and miniSOG

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 77-465

Plasmid

#45970

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 77-465 (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (WT)

Plasmid

#175419

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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miniSOG-Display-5

Plasmid

#54822

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Use

Tags

miniSOG-Display

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#45974

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_Neo_FKBPF36V_mScarlet

Plasmid

#163875

Purpose

Acute Depletion of BRG1-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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CFP_Rem_pcDNA4_HisMaxC

Plasmid

#41651

Depositor

Henry Colecraft

Article

Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

Insert

Rem, GTP binding proteins (RGK) (Rem1 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-TAPP1-PH

Plasmid

#67675

Purpose

Multisite gateway entry clone for adding C-terminal fusions of TAPP1-PH

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

TAPP1-PH

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin C431S

Plasmid

#45976

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin C431S (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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NET39 mRFP-C1 (1219)

Plasmid

#62042

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-mBcl11b-MAR4

Plasmid

#178188

Purpose

Lucifease reporter driven by MAR4 regulatory sequences of mouse Bcl11b

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2018 Mar 20;22(12):3142-3151.

Insert

Bcl11b MAR4

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40 promoter

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pV1386

Plasmid

#111445

Purpose

Solo vector pV1382 + sgScADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F463A

Plasmid

#45975

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F463A (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSG-3'Hu-RelA

Plasmid

#27276

Depositor

Thomas Gilmore

Article

Morin et al Nucleic Acids Res. 1993 May 11. 21(9):21

Insert

RelA (RELA Human)

Use

Tags

GAL4 (aa1-147)

Expression

Mammalian

Mutation

Contains only the transactivation domain (aa 292-…

Promoter

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only