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Showing: 5501 - 5520 of 16077 results

  1. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5ā€²-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  5. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bamā€¦
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

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  12. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    127177-plasmid-map-sequence-id-246566

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  13. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprinā€¦
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

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  14. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argiā€¦
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  15. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  16. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

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  17. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  18. pMLLCK-J72030

    Plasmid
    #26172
    Depositor
    Christopher Anderson
    Insert
    C terminal E tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26172-plasmid-map-sequence-id-12293

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  19. pMLLCK-K112507

    Plasmid
    #26174
    Depositor
    Christopher Anderson
    Insert
    C terminal HA tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26174-plasmid-map-sequence-id-12295

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  20. pMLLCK-J72032

    Plasmid
    #26177
    Depositor
    Christopher Anderson
    Insert
    C terminal S tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26177-plasmid-map-sequence-id-12298

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Showing: 5501 - 5520 of 16077 results