Addgene

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pRha-tns

Plasmid

#170630

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTnsABC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Use

Tags

His6 and TEV site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Article

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

MSCV LTR promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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LV-YFP

Plasmid

#26000

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-YFP (H2BC21 A. victoria, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD

Plasmid

#154938

Purpose

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LV-RFP

Plasmid

#26001

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-RFP (H2BC21 A. victoria, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP_TOMM20_sfCherry2(11)

Plasmid

#83033

Purpose

Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

TOMM20_sfCherry+11

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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