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Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorInsertAbeta(MC1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-4-BE
Plasmid#135019PurposeC-T base editing using LjCDA1L1_4 fused to N terminal of nCas9DepositorInsertLjCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCaSpeR-act5cB-LexAQFw
Plasmid#61311PurposeExpresses chimeric transactivator LexA_DBD:QF2w_AD in DrosophilaDepositorInsertLexA_DBD::QF2w_AD
UseTagsExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP1-1st WW mutant
Plasmid#19004DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEQ1007
Plasmid#83938Purposeexpress human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cellsDepositorInsertIRS1
UseTags6xHISExpressionMammalianMutationR833L in IRS1 (please see depositor comment)PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGR-L3S2P00
Plasmid#46005DepositorInsertL3S2P00 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70
Plasmid#46124DepositorInsertLexABD-G4MD-QFAD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMPO 54
Plasmid#69865PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-MCSII, M13 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sus1/PET32a
Plasmid#26963DepositorInsertSus1 (SUS1 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationwith TEV site to cleave the N-terminal his+Trx tagPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19_TTE1564
Plasmid#61000PurposeT7-transcription template for Tte mRNA used for in vitro translationDepositorInsertTTE1564 transcript
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGR-L3S2P36
Plasmid#46007DepositorInsertL3S2P36 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-NIR-FbLAG30
Plasmid#220748PurposeNIR-FbLAG30 expression in bacteria.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionBacterialMutationFor bacterial cytoplasmic expression, the pelB si…PromoterT7Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
BmTyr-Flag
Plasmid#220779Purposenon-targeted BmTyr throughout the cytoplasmDepositorInsertBacillus megaterium Tyrosinase
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK484 (2mAID-Hygro)
Plasmid#214396Purpose2mAID for N-terminal taggingDepositorInsert2mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK485 (3mAID-Hygro)
Plasmid#214397Purpose3mAID for N-terminal taggingDepositorInsert3mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK454 (dTAG-BSD)
Plasmid#214384PurposedTAG for C-terminal taggingDepositorInsertdTAG-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
G421C_BattB(GA)_mCherry
Plasmid#200633PurposeStrong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing padDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only