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miniSOG-Lifeact-7

Plasmid

#54823

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Use

Tags

Lifeact and miniSOG

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHH0103_PFN2_Profilin

Plasmid

#110007

Purpose

Protein expression and purification of PFN2_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN2_Profilin (PFN2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Y261A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

stop at beginning of last exon

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCB004

Plasmid

#134147

Purpose

Bacterial expression of human Ska3

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

human Ska3 (SKA3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_A0503

Plasmid

#91013

Purpose

Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-Halo donor

Plasmid

#197866

Purpose

Donor plasmid used for building MED6-Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

Halotag

Expression

Mutation

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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MTK0_014

Plasmid

#123929

Purpose

Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBIattB destination vector

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PIGAZFN-R2

Plasmid

#37800

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger R2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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MTK678_002

Plasmid

#123923

Purpose

Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BAC-AmpR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A4110

Plasmid

#91056

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dTaCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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Dlx6

Plasmid

#15539

Depositor

John Rubenstein

Article

Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

Insert

Dlx6 (Dlx6 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
PIGAZFN-L2

Plasmid

#37798

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0512

Plasmid

#124003

Purpose

Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_027

Plasmid

#123742

Purpose

Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VP16::PYLcs-T2A-GAL4DBD::ABIcs

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP-NS-MCS

Plasmid

#75180

Purpose

EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP-NS-MCS

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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