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Plasmid#67542PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pLZ12Km2-P23R:TA:fflucRT
Plasmid#88901PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + Red-shifted firefly luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP2375
Plasmid#127581PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GalToxBFP
Plasmid#68073Purposemammalian expression of GC localized oxBFPDepositorInsertGalT signal anchor transmembrane domain
UseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2350
Plasmid#127580PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH103
Plasmid#84461PurposepJB38-NWMN29-30 + SarA_P1-YFP-TermDepositorInsertSarA_P1-YFP from pTH3
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein
Plasmid#208378PurposeEpisomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split inteinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR
Plasmid#208379PurposeEpisomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistanceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRF-PsrA
Plasmid#49368DepositorInsertPsrA repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BN
Plasmid#60518PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RN
Plasmid#60519PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRF-SrpR
Plasmid#49372DepositorInsertSrpR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNSEN-d2
Plasmid#59763PurposeExpresses nuclear EGFP and Tomato in mammalian cellsDepositorInsertsd2-EGFP
Tomato
UseTags3X NLS and PESTExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH101
Plasmid#84459PurposepJB38-NWMN29-30 + SarA_P1-DsRed-TermDepositorInsertSarA_P1-DsRED from pTH1
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p8266 LentiCRISPR v2 hygro sgNT-1
Plasmid#193977PurposeExpression of spCas9 and non-targeting control sgRNADepositorInsertspCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-ddIPpoI
Plasmid#49052PurposeExpresses the restriction enzyme under the control of Shield1DepositorInsertDD-HA-ER-IPpoI
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorInsertsCopGFP
mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRF-PhlF
Plasmid#49367DepositorInsertPhlF repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-myrGFP
Plasmid#115502Purposeexpression of myristoylated, plasma membrane targeted GFPDepositorInsertmyristoylated GFP
UseTagsExpressionMammalianMutationPromoterchicken beta-actinAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only