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Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
UseTags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6f
Plasmid#207856PurposeMammalian expression of PE6f prime editorDepositorInsertPE6f
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK 4xMito-mEmerald
Plasmid#200430PurposeMammalian expression of fluorescently-tagged marker for mitochondriaDepositorInsert4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
UseTagsmEmeraldExpressionMammalianMutation4x mito-targeting sequencePromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mt-mKeima-P2A-FRB-Fis1
Plasmid#135295Purposestably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cellsDepositorInsertmt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
UseLentiviralTagsExpressionMammalianMutationmt-mKeima is fused with FRB-Fis1 via P2A seqeuncePromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSExpressionMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGFPGUSPlus
Plasmid#64401Purposebinary plant vector expressing GFP and GUS, both driven by a 35S promoterDepositorInsertsGFP
GUSPlus
UseBinary vector for plant transformationTagsHisExpressionPlantMutationS65TPromoter35SAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF1
Plasmid#53209PurposeExpressed N-terminally Hexahistidine tagged TRF1 proteinDepositorInsertTRF1 (TERF1 Human)
UseTagsHexahistidineExpressionBacterialMutation3-439PromoterTrcAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0608
Plasmid#142659PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAFA (MAFA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5059
Plasmid#198154Purposebacterial expression of steC::HaloTag fusionDepositorInsertPsteC steC::L16::HaloTag::HA
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV2)
Viral Prep#50476-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tomm20-ECFP-RspANFAST
Plasmid#216348Purposetargets ECFP-RspANFAST to mitochondrial membraneDepositorInserttomm20-ECFP-RspANFAST
UseTagstomm20ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJF143.J3
Plasmid#153028PurposeJ3-mRFP1DepositorInsertJ3-mRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluA1 (px330:2-guideCas9)
Plasmid#169417PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEX-GFP-hLC3WT
Plasmid#24987DepositorInserta wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only