Addgene

We narrowed to 11,160 results for: cel.2

Showing: 5441 - 5460 of 11160 results

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pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Zyxin-6

Plasmid

#55101

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Use

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZIBG2

Plasmid

#169458

Purpose

cell surface localized zinc sensor with ~ 300 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

Insert

ZIBG2

Use

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Expression

Mutation

Promoter

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Use

Tags

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKS2311

Plasmid

#37835

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)

Use

Tags

YFP

Expression

Worm

Mutation

W32A;Y35A

Promoter

nhx-2

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-Y-GECO2m

Plasmid

#105070

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.2s

Plasmid

#105069

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2m

Plasmid

#105066

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2s

Plasmid

#105067

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.1s

Plasmid

#105068

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.1s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54(L53P,L67P)

Plasmid

#172458

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Leucines 53 and 67 to proline (breaks coi…

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH1-FLAG

Plasmid

#66802

Purpose

Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-FLEX-XRI-FLAG

Plasmid

#178057

Purpose

Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

XRI-FLAG

Use

AAV

Tags

FLAG-MBP

Expression

Mammalian

Mutation

Promoter

Human ubiquitin C (UBC) promoter

Available since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-GFP

Plasmid

#72895

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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