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Plasmid#182994Purposeexpression of minitalin wt in mammalian cellsDepositorInsertminitalin (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
talin1-head
Plasmid#182996Purposeexpression of talin1 head wt in mammalian cellsDepositorInserttalin1 head (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-NAD
Plasmid#193824PurposeCMV overexpression of ChemoB-NAD in mammalian cell linesDepositorInsertChemoB-NAD
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Firefly
Plasmid#79495PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDSDepositorInsertFirefly Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRbm10/Cre
Plasmid#89648PurposeExpresses an Rbm10-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Rbm10 (Rbm10 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA P2
Plasmid#80944PurposeExpresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 bindingDepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK-E3mut
Plasmid#29691DepositorInsertGFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
UseTagsFlag and GFPExpressionMammalianMutationrecognition sites only, and E3 ligase site mutatedPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx2(yeast)
Plasmid#65005PurposeYeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2DepositorInsertGRX2
UseTagsroGFP2ExpressionYeastMutationPromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-ML1N*2(7Q)
Plasmid#67679PurposeMultisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)DepositorInsertML1N*2(7Q)
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-vHras-zeo
Plasmid#17756DepositorInsertvHras (genomic0 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationV12DPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-THT III
Plasmid#55791PurposeGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistanceDepositorTypeEmpty backboneUseRNAi; GatewayTagsExpressionMammalianMutationPromotermodified H1 RNA geneAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-dKeNLuc_SV40:PuroR
Plasmid#183046PurposeExpresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.DepositorInsertdKeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc_SV40:PuroR
Plasmid#183047PurposeExpresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE662
Plasmid#153260PurposeM6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE664
Plasmid#153262PurposeM7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE659
Plasmid#153257PurposeM4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only