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Plasmid#199318Purposemutated phosphorylation site (MLKL interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to AlaninePromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
COC053
Plasmid#209131PurposeEnzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.DepositorInsertEnzyme BirA (birA E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2
Plasmid#135412Purposesimultaneous optical recording and manipulation of electrical activity in neuronsDepositorInsertSomArchon-EGFP-P2A-stGtACR2
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2 CRISPR
Plasmid#140649PurposeSMC2 tagging CRISPRDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
Plasmid#100279PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutantDepositorInsertKdm6b (Kdm6b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationMutated catalytic domain (1025aa-End) (please see…PromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorInsertSTAR protein (STAR Human)
UseTagsHis-tagged MBPExpressionBacterialMutationPromoterPtacAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S199D][S227D]-FLAG
Plasmid#199324Purposedouble mutated phosphorylation site (kinase acitivity and MLKL interaction active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 199 and 227 to Aspartic AcidPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD442
Plasmid#153024PurposedCas9, MCP-SoxS(R93A/S101A)DepositorInsertdCas9, MCP-SoxS(R93A/S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFAB3677
Plasmid#47833PurposeBIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.DepositorInsertpromoter 2 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV6- Entry-RPL26DC-DDK
Plasmid#133867PurposeExpression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sitesDepositorInsertRPL26DC-myc-FLAG (RPL26 Human)
UseTagsMyc-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo
Plasmid#78352PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHM024Env
Plasmid#137691PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S199A][S227A]-FLAG
Plasmid#199323Purposedouble mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 199 and 227 to AlaninePromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only