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Plasmid#78539PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.3
Plasmid#78538PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Exon.3
Plasmid#78542PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.2
Plasmid#78537PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT
Plasmid#61796PurposeLuciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutatedDepositorInsertmouse Ywhab 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT
Plasmid#61793PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutatedDepositorInsertmouse Xbp1 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT
Plasmid#61791PurposeLuciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutatedDepositorInsertmouse Smad5 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT
Plasmid#61790PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutatedDepositorInsertmouse Map3k1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseTagsExpressionMutationboth miR-200c MRE-2 and MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT
Plasmid#61786PurposeLuciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutatedDepositorInsertmouse Crtap 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLexA::SRDX (GB0732)
Plasmid#68184PurposeProvides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLexA Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR11
Plasmid#69154Purposeread-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IVDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterfzr-1 and rps-27Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pES006 (pTet-qacR 2/pQacA-deGFP)
Plasmid#69035Purposereporter with qacR 2 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationE57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…PromoterpQacA and pTetAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD::BDR (GB0734)
Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL836
Plasmid#62827PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1DepositorInsertsgfp
ipex leader
hfq leader
UseSynthetic BiologyTagsompC leader and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2107
Plasmid#62841PurposeLim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsm8::micC leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialMutationPromoterpConNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238) in pcDNAI/Amp
Plasmid#60896PurposeThis vector attaches CFP(159-238) to the N-terminus of a protein.DepositorInsertCFP(159-238)
UseTagsExpressionMammalianMutationAsn-164 to HisPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT
Plasmid#61787PurposeLuciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutatedDepositorInsertmouse Fhod1 3'UTR with miR-200c MRE-2 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-2 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT
Plasmid#61788PurposeLuciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutatedDepositorInsertmouse Tob1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only