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Addgene
Showing: 5401 - 5420 of 16077 results

  1. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

    Enlarge

  2. pENTR-R2L3-cmER-IRESpuro-pA

    Plasmid
    #139539
    Purpose
    Provides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    139539-plasmid-map-sequence-id-274701

    Enlarge

  3. Nesprin3-C-18

    Plasmid
    #58194
    Purpose
    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pIC81

    Plasmid
    #63065
    Purpose
    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    PMFI, IMAGE clone 6148250 (PMF1 Human)
    Use
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63065-plasmid-map-sequence-id-104380

    Enlarge

  5. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Availability
    Academic Institutions and Nonprofits only

  9. pLenti-CMV-paGFP-bPAC

    Plasmid
    #204670
    Purpose
    Mammalian expression of bPAC tagged with paGFP for cAMP production
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Insert
    paGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    204670-plasmid-map-sequence-id-407792

    Enlarge

  13. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

    Enlarge

  14. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

    Enlarge

  15. pET28b(+)-TM0848

    Plasmid
    #67287
    Purpose
    Expresses TM0848 in E. coli
    Depositor
    SeWon Suh
    Insert
    GTP-binding protein YqeH
    Use
    Tags
    N tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  16. pMLLCK-J72029

    Plasmid
    #26171
    Depositor
    Christopher Anderson
    Insert
    C terminal avi tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26171-plasmid-map-sequence-id-12292

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  17. plex_307-mKate-ccdB-g418

    Plasmid
    #201125
    Purpose
    pLEX_307 plasmid with N-terminal mKate tag and g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    mKate
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Availability
    Academic Institutions and Nonprofits only
    201125-plasmid-map-sequence-id-397034

    Enlarge

  18. plex_307-EYFP-ccdB-g418

    Plasmid
    #201128
    Purpose
    pLEX_307 plasmid with N-terminal EYFP tag and g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Availability
    Academic Institutions and Nonprofits only
    201128-plasmid-map-sequence-id-396318

    Enlarge

  19. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  20. PSMA4_BirA_Cterm

    Plasmid
    #221513
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psma4
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221513-plasmid-map-sequence-id-439223

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Showing: 5401 - 5420 of 16077 results