Addgene

We narrowed to 14,618 results for: jun

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pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179038

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-CTAP-PrP-SBP_W

Plasmid

#147835

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-PelA

Plasmid

#170722

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Pectin lyase A

Use

Gateway donor vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207-MUM2sp-RglB

Plasmid

#170721

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Rhamnogalacturonan lyase B

Use

Gateway donor vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207-MUM2sp-RglA

Plasmid

#170720

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Rhamnogalacturonan lyase A

Use

Gateway donor vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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DDX41_p.L237F_p.P238T_EGFP

Plasmid

#175845

Purpose

Expresses DDX41 with mutation

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

c.711G>T, p.L237F and c.712C>A, p.P238T

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#3

Plasmid

#169791

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#2

Plasmid

#169790

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#1

Plasmid

#170834

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#2

Plasmid

#170838

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Limk1 gRNA#3

Plasmid

#163393

Purpose

Cas9-mediated knockout of Limk1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#3

Plasmid

#170839

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#1

Plasmid

#170837

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Limk1 gRNA#2

Plasmid

#163392

Purpose

Cas9-mediated knockout of Limk1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#1

Plasmid

#169789

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Venus-Actb

Plasmid

#169788

Purpose

An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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