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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LZF40: LZF40_Fsyn_Cre

Plasmid

#109378

Purpose

Synaptic transmission

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

Cre-NCBI

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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sgALK(Chr. Inv.)

Plasmid

#85569

Purpose

Expresses sgRNA targeting human ALK between exon 20 and 21

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Tags

Expression

Mutation

Promoter

R07E5.16 (U6)

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pVZ_PcoaT_SQE1

Plasmid

#103860

Purpose

Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803

Depositor

Ilka Axmann

Article

Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

Insert

squalene epoxidase 1 (XF1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted gene is codon optimized. 2nd amino acid …

Promoter

PcoaT (from Synechocystis sp. PCC 6803)

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763)-FLAG (no His-tag)

Plasmid

#96887

Purpose

expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Use

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36

Promoter

T7

Available since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgEGFR(B)

Plasmid

#85565

Purpose

Expresses sgRNA targeting human EGFR around codon T790

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

epidermal growth factor receptor (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pCDF-casBCDE(+6)

Plasmid

#89728

Purpose

Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

Depositor

Konstantin Kuznedelov, Konstantin Severinov

Article

Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Derivative CRISPR array containing a longer versi…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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3' MBD3 TALEN

Plasmid

#52359

Purpose

TALEN vector for targeting human MBD3 locus (3')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

mEGFP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264-SIM1_mut-cFlag

Plasmid

#220205

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 NSP5_K90R

Plasmid

#194819

Purpose

Sars-CoV-2 NSP5_K90R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP5_K90R (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

K90R

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP6_S107-109del

Plasmid

#194820

Purpose

Sars-CoV-2 NSP6_S107-109del in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP6_S107-109del (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

S107-109del

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 ORF3a_Q57H

Plasmid

#194821

Purpose

Sars-CoV-2 ORF3a_Q57H in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

ORF3a_Q57H (ORF3a Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

Q57H

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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