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Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MLS-HyPer7
Plasmid#136470PurposeMammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTandem mitochondrial targeting signal of cytochro…ExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FAK-5
Plasmid#55044PurposeLocalization: Focal adhesion kinase, Excitation: 587, Emission: 610DepositorInsertFAK
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKH3-human RSK1
Plasmid#13841DepositorInsertRSK1 (RPS6KA1 Human)
UseTags3x HAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA p21
Plasmid#78782PurposeTo overexpress p21 in Mammalian CellDepositorInsertp21 (CDKN1A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
CX3CR1-EGFP
Plasmid#24061DepositorInsertCX3CR1 (Cx3cr1 Mouse)
UseMouse TargetingTagsEGFPExpressionMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 alpha6
Plasmid#16036DepositorInsertalpha6 (ITGA6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-SpyCas9
Plasmid#113033PurposeSpyCas9 expression in mammalian cells (CMV promoter driven)DepositorInsertSpyCas9
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ:RUBY
Plasmid#160909PurposeRUBY under the control of Ubiquitin promoter/marker for transformationDepositorInsertRUBY and UBIQUITIN promoter
UseTagsExpressionPlantMutationPromoterUBIQUITINAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2
Plasmid#161753PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.DepositorInsertExRai-AKAR2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLH-stsgRNA3.1
Plasmid#64118PurposeVector for expression of St1 sgRNA3.1 in mammalian cellsDepositorInsertSt1 sgRNA3.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-Wasabi
Plasmid#86117PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38DepositorInsertsTcI38
mWasabi
UseTags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterpR/pL and pR/pL, LacIAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#226514PurposeMamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2DepositorInsertHaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)
Viral Prep#73178-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.DepositorPromoterTagsNoneAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only