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Plasmid#64945PurposeFor retroviral expression of E3 ubiquitin ligase osTIR1 with 9x myc tagDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseRetroviralTags9x MycExpressionMammalianMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAX2
Plasmid#117398Purposeallelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switchDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
hRIP3 GFP D160N
Plasmid#41386DepositorInsertRIP3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationKinase Domain Mutant (D160N)PromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPP394
Plasmid#158800PurposePlasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PL03-mVenus_tetR
Plasmid#137663PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.DepositorInsertPL03:mVenus
UseTagsExpressionBacterialMutationPromoterPL03Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA/Myc DeltaN TCF4
Plasmid#16513DepositorInsertTCF4 (TCF7L2 Human)
UseTagsExpressionMammalianMutationDelta N: lacks aa 1-32PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RFP-p2a-postASAP
Plasmid#178793PurposeExpresses voltage sensor targeted to dendrites and spines along with RFPDepositorInsertFingR.PSD95, ASAP2f and TagRFP
UseTagsTagRFPExpressionMammalianMutationmutations in amino acids L146G, S147T N149R, S150…PromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p4RTK GL4.10 (Luc2)
Plasmid#16057DepositorInsertMultimerized E-box
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJZC545
Plasmid#62313PurposesgRNA with 1x MS2 for yeast cellsDepositorInsertsgRNA + 1x MS2 binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT376
Plasmid#125617PurposeExpresses BE4max-NG in mammalian cellsDepositorInsertBE4max-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW170b2
Plasmid#136929PurposeDirected evolution of SpCas9DepositorInsertgVI
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreen_dualluc_ORF_sensor
Plasmid#55207PurposeDual-luciferase reporter for microRNA targeting in plantsDepositorInsertsRenilla luciferase
Firefly luciferase
UseLuciferase; Plant transient expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD011
Plasmid#116852PurposeExpresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011DepositorInsertsAraC
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP
Plasmid#84254PurposeExpresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
hRIP3 GFP RHIM mut
Plasmid#41385DepositorInsertRIP3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationRHIM mutant (AAAA-459-462)PromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only