Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 5381 - 5400 of 16077 results

  2. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid
    #138299
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKCsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138299-plasmid-map-sequence-id-307463

    Enlarge

  3. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid
    #136447
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Use
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-716). Codon-optimize…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    136447-plasmid-map-sequence-id-273651

    Enlarge

  8. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

    Enlarge

  9. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

    Enlarge

  10. pE-mApple L5L2

    Plasmid
    #187690
    Purpose
    C-Term Gateway cloning tag
    Depositor
    Mary Beckerle
    Insert
    mApple
    Use
    Gateway entry vector
    Tags
    mApple
    Expression
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    187690-plasmid-map-sequence-id-371407

    Enlarge

  11. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Use
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

    Enlarge

  12. pNIC28-BIRC7

    Plasmid
    #196363
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC7/Livin
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    196363-plasmid-map-sequence-id-386127

    Enlarge

  13. pNIC28-BIRC3

    Plasmid
    #196366
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC3/cIAP2
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    196366-plasmid-map-sequence-id-386071

    Enlarge

  14. plex_307-3xflag-ccdb-puro

    Plasmid
    #201122
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and puro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Availability
    Academic Institutions and Nonprofits only
    201122-plasmid-map-sequence-id-395513

    Enlarge

  15. PSMC2_GFP_Cterm

    Plasmid
    #221516
    Purpose
    Fluorescently tagged Psmc2-GFP
    Depositor
    Alessandro Ori
    Insert
    PSMC2 (PSMC2 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221516-plasmid-map-sequence-id-439441

    Enlarge

  16. pBEN1-SGC

    Plasmid
    #39127
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Tags
    Strep tag and SET1
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

    Enlarge

  18. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Industry, Academic Institutions, and Nonprofits
    26096-plasmid-map-sequence-id-326568

    Enlarge

  19. pN1-OPA1(123Nt)-Halo

    Plasmid
    #129345
    Purpose
    Halo Tag, Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    Halo (Promega)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129345-plasmid-map-sequence-id-260109

    Enlarge

  20. pMSCV-FLAG-puro-Mock

    Plasmid
    #206911
    Purpose
    pMSCV backbone with FLAG tag for protein expression and puromycin selection
    Depositor
    Hyonchol Jang
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206911-plasmid-map-sequence-id-410076

    Enlarge

Showing: 5381 - 5400 of 16077 results