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Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(+10A)-IDR-mCherry
Plasmid#145287PurposeBacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion ProteinDepositorInsertRUNX2(+10A)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB3
Plasmid#63104PurposeExpression of HSPB3 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb3 (HSPB3 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-EOMESwt-IDR
Plasmid#145231PurposeExpresses fusion of GAL4 DNA-binding domain and EOMESwt IDRDepositorInsertEOMESwt-IDR (EOMES Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41wt-IDR
Plasmid#145229PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDRDepositorInsertBHLHE41wt-IDR (BHLHE41 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13wt-IDR
Plasmid#145240PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13wt IDRDepositorInsertHOXA13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-T0-eGFP-HROB
Plasmid#135299PurposeExpresses eGFP-tagged HROB in mammalian cellsDepositorInsertHROB (HROB Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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p-OiRS3GG
Plasmid#98589PurposeExpresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertsgroup I intron
PheS cassette to be selectively replaced by unique asRNA probes
UseTagsExpressionBacterialMutationPromoterpBAD and pLtetOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271E
Plasmid#73057PurposeC-terminal GFP fusion of Mouse Foxg1 T271EDepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271EPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271A
Plasmid#73047PurposeC-terminal fusion of gfp Mouse Foxg1 T271ADepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271APromoterCAGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-I560S-mDPYD
Plasmid#61615PurposeThe mouse Dpyd was used to rescue the human DPYD hairpin affect on EMTDepositorInsertDihydropyrimidine dehydrogenase (Dpyd Mouse)
UseTagsExpressionMammalianMutationChanged Isoleucine at position 560 to SerinePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-HA-p31(comet)-S102A
Plasmid#221698PurposeMammalian expression of HA-tagged human p31(comet) (S102A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationS102APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-R42A
Plasmid#221702PurposeLentiviral expression of human RNF8 (R42A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-C403S
Plasmid#221703PurposeLentiviral expression of human RNF8 (C403S) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-S157A
Plasmid#221704PurposeLentiviral expression of human RNF8 (S157A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationS157APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8
Plasmid#221676PurposeMammalian expression of Myc-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD090A
Plasmid#218132PurposeHybrid circuit displaying mmCRBN bait and driving expression of luxABDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD138A
Plasmid#218130PurposeHybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxABDepositorInsertsUseTagsscP22ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only