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Plasmid#188995PurposeA mammalian expression plasmid encoding miRFP-tagged human OCT1DepositorInserthOCT1 (SLC22A1 Human)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVG48
Plasmid#164706PurposertTA under the control of PGK1 promoterDepositorInsertPGK1pr - rtTA - CYC1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-DHFR
Plasmid#20217DepositorInsertDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAH2
Plasmid#41826PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
U6-U57C
Plasmid#169928PurposeWT U6 with a U57C mutationDepositorInsertU6 U57C
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAH1
Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Vh-HA-3xDHFR
Plasmid#20219DepositorInsert3xDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 WT
Plasmid#87599PurposeEncodes Prp5 WT for expression in yeastDepositorInsertPRP5 (PRP5 )
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-2xDHFR
Plasmid#20218DepositorInsert2xDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-dCer3
Plasmid#191328PurposeExpresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membraneDepositorInsertdCer3-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mScarlet
Plasmid#191324PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mScarlet (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTG…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG-02
Plasmid#166933PurposeTarget AD1.A5 part (G)DepositorInsertStar sense AD1.S5 +RBS - Block G- Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-03
Plasmid#166927PurposeSTAR-AD1.A6 part (C)DepositorInsertStar Antisense AD1.A6 - Block C - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-02
Plasmid#166926PurposeSTAR-AD1.A5 part (C)DepositorInsertStar Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG50
Plasmid#164708PurposeyEVenus under the control of tetOpr with ADH1t upstream of the reporterDepositorInsertADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only