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We narrowed to 4,676 results for: puc

Showing: 521 - 540 of 4676 results

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pUC19-hAPOE-R-loop

Plasmid

#134901

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

APOE promoter (APOE Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-Mu-switch-R-loop

Plasmid

#134899

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mouse IgH switch repeat region

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2-GFP

Plasmid

#202194

Purpose

knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP knock-in

Expression

Mutation

Promoter

lac

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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IMBB2.4-pUC57-mini

Plasmid

#88845

Purpose

plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

Type

Empty backbone

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-MCC

Plasmid

#133307

Purpose

"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

microcin-V bacteriocin cassette (cvaC E. coli)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N112D (please see depositors comments)

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pUC118-FLIP-Puro

Plasmid

#84538

Purpose

a mock vector for generating FLIP targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP - Puro

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pUC57-Amp_CRISPIE donor B5

Plasmid

#172846

Purpose

CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18R6K-mini-Tn7T

Plasmid

#64958

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19 Giles integrase

Plasmid

#170049

Purpose

pUC19 with Giles integrase

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Insert

GIles Integrase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7

Plasmid

#64953

Purpose

mini-Tn7 base vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18T-mini-Tn7T-Tp

Plasmid

#65024

Purpose

TpR mini-Tn7 vector, mobilizable

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-white[coffee]

Plasmid

#84006

Purpose

Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Insert

A 2,080 bp fragment of the white[coffee] allele (w Fly)

Use

Unspecified

Tags

Expression

Mutation

A GC-to-AA mutation that creates a G589E missense…

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-GW

Plasmid

#64961

Purpose

mini-Tn7 Gateway destination vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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