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Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYP1
Plasmid#202406PurposeBackbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterfdeAAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DJ-N589X
Plasmid#130878PurposeEncodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codonDepositorInsertAAV-DJ cap N589X
UseTagsExpressionMammalianMutationN589XPromoterUnknownAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDA010.EL222
Plasmid#206801PurposeJ23106.EL222DepositorInsertEL222
UseTagsExpressionMutationPromoterJ23106Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X
Plasmid#182365PurposeExpresses Neq2X DNA polymeraseDepositorInsertNeq2X
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXY027
Plasmid#98915PurposeExpress FtsZ tagged with GFP in E coliDepositorInsertFtsZ
UseTagsGFPExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR eGFPCreERT2 L1-R5
Plasmid#43961DepositorInserteGFP-CreERT2
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDA040.BLD7-mRFP
Plasmid#206800PurposeEL222_D7_mRFPDepositorInsertBLD7.mRFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-lox2272
Plasmid#154833PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanerceptDepositorInsertETN
UseCre/LoxTagsExpressionMammalianMutationPromoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-eSIBR
Plasmid#71664PurposeEGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backboneDepositorInsertEGFP coding sequence followed by eSIBR cassette
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Spec
Plasmid#104521PurposeLI resistance gene moduleDepositorInsertSpectinomycin resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA2[PLac:GFP]
Plasmid#60778PurposeContains Plac driving expression of GFPDepositorInsertyemGFP
UseTagsExpressionMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT009.J3.RFP
Plasmid#177324PurposeActivatable RFP expressionDepositorInsertmRFP1
UseTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Kan
Plasmid#104523PurposeLI resistance gene moduleDepositorInsertKanamycin resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only