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Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX429 SpCas9 N863A nickase
Plasmid#68706PurposeSpCas9 N863A nickaseDepositorInsertSpCas9 N863A nicking mutant
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-Δ(325-355)
Plasmid#24871DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationDeletion of aa 325-355PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(310-360)
Plasmid#24872DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains only the dimeric oligomerization domain …PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-N268D
Plasmid#24861DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationAsparagine 268 mutated to AspartatePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGENE:EFF-1::mCherry::V5
Plasmid#133020PurposeEFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cellsDepositorInsertEFF-1::mCherry::V5
UseTagsV5; mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-L344P
Plasmid#24862DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationLeucine 344 mutated to ProlinePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-M340Q-L344R
Plasmid#24863DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationMethionine 340 mutated to Glutamine Leucine 344 …PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
PX544 SpCas9(del1098-1368)
Plasmid#58889PurposeTruncated form of SpCas9 without C' domainDepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 1098-1368PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS6_4323_v1
Plasmid#126859Purpose4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7DepositorInsertIV_CS6_4323_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE3-VP64-P10
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX543 SpCas9(del175-307)
Plasmid#58888PurposeTruncated form of SpCas9 without REC2 domainDepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 175-307PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE1-VP64-P10
Plasmid#104606PurposeExpresses TALE1 under UAS controlDepositorInsertTALE1
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE4-VP64-P10
Plasmid#104608PurposeExpresses TALE4 under UAS controlDepositorInsertTALE4
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K403A
Plasmid#195612PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA1463G, A1464CPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Q22YU3-KO
Plasmid#170313PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selectionDepositorInsertQ22YU3 knockout
UseTetrahymena somatic gene knockout vectorTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Q22MS1-KO
Plasmid#170312PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selectionDepositorInsertQ22MS1 knockout
UseTetrahymena somatic gene knockout vectorTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22MS1-2xStrep
Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only