Addgene

We narrowed to 2,327 results for: Xpa

Showing: 521 - 540 of 2327 results

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pHJ2: LexA DBD

Plasmid

#108240

Purpose

LexA DNA binding domain

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain (1-87)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2375

Plasmid

#67546

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2224

Plasmid

#67547

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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C-1-BE

Plasmid

#135029

Purpose

C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

PmCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ1_Full length LexA

Plasmid

#107237

Purpose

sfGFP expression repressed by transcriptional factor LexA

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Full length LexA repressor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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SaMscL (CDelta26)

Plasmid

#79027

Purpose

Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

Depositor

Douglas Rees

Article

Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

Insert

MscL

Use

Tags

6x Histidine Tag

Expression

Mutation

Stop codon placed before 26 amino acids at C Term…

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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N-4-BE

Plasmid

#135019

Purpose

C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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PILAR

Plasmid

#22851

Depositor

Jose Conejo-Garcia

Article

Huarte et al Blood. 2008 Aug 15. 112(4):1259-68.

Insert

PILAR (CLEC2A Human)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only
pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70

Plasmid

#46124

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB2223

Plasmid

#67544

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLei-wt-sfGFP

Plasmid

#191113

Purpose

An E. coli sfGFP wild type control

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-MfnG-TEV-His

Plasmid

#191114

Purpose

Expresses MfnG-TEV-His in E. coli

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK484 (2mAID-Hygro)

Plasmid

#214396

Purpose

2mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

2mAID-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK485 (3mAID-Hygro)

Plasmid

#214397

Purpose

3mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

3mAID-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK454 (dTAG-BSD)

Plasmid

#214384

Purpose

dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

dTAG-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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