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Plasmid#89953PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.DepositorInsertrpsL gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ldhA
Plasmid#102288PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.DepositorInsertldhA gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-malG
Plasmid#89951PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.DepositorInsertmalG gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-hisG
Plasmid#89954PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.DepositorInserthisG gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-trmI
Plasmid#89955PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.DepositorInserttrmI gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-xylA
Plasmid#89964PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.DepositorInsertxylA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-lpoB
Plasmid#89959PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.DepositorInsertlpoB gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-fsaA
Plasmid#102286PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.DepositorInsertfsaA gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-adhE
Plasmid#102290PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.DepositorInsertadhE gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ompT
Plasmid#102291PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.DepositorInsertompT gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-srlA
Plasmid#89965PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.DepositorInsertsrlA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rbsK
Plasmid#89963PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.DepositorInsertrbsK gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-yhhT
Plasmid#89958PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.DepositorInsertyhhT gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rssA
Plasmid#89957PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.DepositorInsertrssA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-torZ
Plasmid#89956PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.DepositorInserttorZ gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-arsB
Plasmid#89952PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.DepositorInsertarsB gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYY_S1_400
Plasmid#170470PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, Os, O1, lambda t1, in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LMB
Plasmid#12502DepositorInsertLMB
UseTags6xHisExpressionBacterialMutationRosettaDesign was used to design sequences. Align…PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-apr
Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only