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Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN3-mTagBFP-pHluorin-CAAX
Plasmid#178345PurposeExpress a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoterDepositorInsertmTagBFP-pHluorin-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-SunTag(10X)-2A-BFP
Plasmid#227389PurposeAdhesion module, dCas9-SunTagDepositorInsertdCas9-SunTag
UseLentiviralTagsExpressionMutationEnzymatically dead Cas9PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
iC13 PB-TO-oNgn2-BFP-puro
Plasmid#204733PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTAGBFP2 Peroxisome visual barcode
Plasmid#158668Purposetagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-mTagBFP2-T2A-iCreERT2
Plasmid#177308PurposeCAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposonDepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL SFFV d20GFP.T2A.mTagBFP Donor
Plasmid#31485DepositorInsertSFFV d20BFP T2A BFP
UseLentiviralTagsExpressionMutationGFP has 20 amino acids deleted from the C terminu…PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W
Plasmid#105036PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorInsertPou5f1 (Pou5f1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk2
Plasmid#50848PurposeFluorescent reporter for Erk nuclear translocationDepositorInsertErk2 (Mapk1 Mouse)
UseLentiviralTagsTagBFPExpressionMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-i53-bpA-EF1BFP
Plasmid#111145PurposeExpression of i53 and a BFP reporter in mammalian cellsDepositorUseTagsExpressionMammalianMutationhuman Ubiquitin, contains 8 point mutationsPromoterCAG promoter and EF1Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorArticleInsertmTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-5xGCN4-P2A-BFP
Plasmid#184438PurposedCas9 fused with 5 copies of GCN4 epitopeDepositorInsertdCas9-5xGCN4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-TagBFP
Plasmid#214721PurposeExpresses green #2.4 pre-SynapShot and TagBFPDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-eBFP2-NLS-pA
Plasmid#183800PurposeAAV vector to express nuclear localized eBFP2 from CMV promoterDepositorInserteBFP2-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TagBFP-hCoilin
Plasmid#182589Purposeexpresses bfp-hcoilin in mammalian cellsDepositorInsertCoilin (COIL Human)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)
Plasmid#172065PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intronDepositorInsertmTagBFP2(11x7)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-Lari-BFP2-TSERex
Plasmid#123311PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertLari-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab7L1-c-Myc
Plasmid#79803PurposeExpress TagBFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits