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Bacterial Strain#195855PurposeE. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
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Human SLC KO Library
Pooled Library#132552PurposeExpresses gRNAs for knockout of the human Solute Carrier familyDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-FnCas9
Plasmid#130969Purpose3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNADepositorInsertFnCas9
UseCRISPRTags3xHA-NLS and NLS-T2A-EGFPExpressionMammalianMutationPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-Selo-FI-prk
Plasmid#162698PurposeExpresses S. elongatus Form IB rubisco and S. elongatus PrkDepositorInsertS. elongatus Form IB rubisco and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWR63(YUM1)
Plasmid#203336Purposeepisomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR63
Plasmid#203331Purposeepisomal Cas9 and sgRNA expression vector without sgRNA for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PBK-PRT100AH
Plasmid#44739DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA
Plasmid#171385PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized minidCas13X.1-RESCUE-S
UseTagsExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166015Purposemammalian expression of HCoV-OC43 soluble spike trimer proteinDepositorInsertHCoV-OC43 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166014Purposemammalian expression of HCoV-HKU1 soluble spike trimer proteinDepositorInsertHCoV-HKU1 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166017Purposemammalian expression of HCoV-NL63 soluble spike trimer proteinDepositorInsertHCoV-NL63 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2
Plasmid#166016Purposemammalian expression of HCoV-229E soluble spike trimer proteinDepositorInsertHCoV-229E S (spike) (S )
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6-MBP TEV Ura S. cerevisiae expression vector (12URA-C)
Plasmid#48305DepositorTypeEmpty backboneUseNaTagsHis6 and MBPExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)
Plasmid#48313DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166013Purposemammalian expression of MERS-CoV soluble spike trimer proteinDepositorInsertMERS-CoV S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA
Plasmid#171384PurposeExpression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized dCas13X.1-RESCUE-S
UseTagsExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166012Purposemammalian expression of SARS-CoV soluble spike trimer proteinDepositorInsertSARS-CoV S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6
Plasmid#166018Purposemammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)DepositorInsertSARS-CoV-2 RBD (S )
UseTagsHis6ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6-MBP TEV Trp S. cerevisiae expression vector (12TRP-C)
Plasmid#48302DepositorTypeEmpty backboneUseNaTagsHis6 and MBPExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only