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Plasmid#182322PurposeActin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-μDronpa-TagRFP-T
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PKA
Plasmid#60936PurposeFluorescent FPX sensor for cAMPDepositorInsertPKA FPX Sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgLenti
Plasmid#105996Purposesingle sgRNA expression vectorDepositorTypeEmpty backboneUseCRISPR and Lentiviral; S.pyogenes sgrna expressio…TagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53-tTR-KRAB
Plasmid#11654DepositorInserthUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
Rix-PGK-Tom-W
Plasmid#25813DepositorInsertRix-PGK-Tom-W
UseLentiviralTagsExpressionMutationtdTomato from Tsien labPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorInsertLyn-RAB-EKARev
UseTagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-NES
Plasmid#118407PurposeCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-NES
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-GDNF-rtTR-KRAB-2SM2
Plasmid#11647DepositorInsertmPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off (GDNF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
UseTagsNuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only