Showing: 5341 - 5360 of 16077 results
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Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorInsertMst1 (STK4 Human)
UseTagsHA, SBPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
UseTagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pVH003
Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPFAS-2XStrep
Plasmid#125682Purposeexpresses human PFAS protein with a C-terminal 2XStrep affinity tagDepositorInsertPFAS (PFAS Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-RLuc8N-NES
Plasmid#138212PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertPKAsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p6H-SUMO3-ORF24-NTD-Strep
Plasmid#138467PurposeExpresses SUMO3-tagged ORF24-NTD-Strep in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsSUMO3 and Strep-Tag IIExpressionBacterialMutationAmino acids 2-201PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev-NLS
Plasmid#118476PurposeddRFP-based single-color ERK biosensor targeted to the nucleus.DepositorInsertRAB-EKARev-NLS
UseTags6xHIS, Nuclear localization signal (NLS), T7 tag …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-Venus-FLARE-Cameleon
Plasmid#123351PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertVenus-Venus-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-Cameleon
Plasmid#123352PurposeRed-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertmCherry-mCherry-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon
Plasmid#123353PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertCerulean3-Cerulean3-FLARE-Cameleon
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2clust
Plasmid#105624PurposeExpresses improved CRY2PHR homo-oligomerizationDepositorInsertmCherry-CRY2PHR-9aa (CRY2 Human, Mustard Weed)
UseTags9 amino acidsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 5341 - 5360 of 16077 results