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Plasmid#145258PurposeMammalian Expression of Opto HOXD13 IDRDepositorInsertHOXD13wt-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:cAPX
Plasmid#84736PurposeSimultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsNLS-SV40ExpressionPlantMutationPromoterpFMV/p35SAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 E17K
Plasmid#73050PurposeHuman AKT3 E17KDepositorInsertAKT3 E17K (AKT3 Human)
UseTagsExpressionMammalianMutationE17KPromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_Clover
Plasmid#135179PurposeAAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(WT)-mCherry
Plasmid#79421PurposeExpresses C-terminally mCherry-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf3(WT)-mCherry
Plasmid#79420PurposeExpresses C-terminally mCherry-tagged Arf3(WT) in mammalian cellsDepositorInsertArf3 (ARF3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo
Plasmid#170319PurposeExpresses HA and EGFP tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Nkx2.5
Plasmid#72689PurposeDoxycyline regulated expression of Nkx2.5 in mammalian cellsDepositorInsertNkx 2.5 (Nkx2-5 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 K177M
Plasmid#73051PurposeHuman AKT3 K177MDepositorInsertAKT3 K177M (AKT3 Human)
UseTagsExpressionMammalianMutationK177MPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2144
Plasmid#104357PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporterDepositorInsertssAAV-smCBA-EmGFP
UseAAVTagsExpressionMutationPromotersmCBAAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 S472A
Plasmid#73052PurposeHuman AKT3 S472ADepositorInsertAKT3 S472A (AKT3 Human)
UseTagsExpressionMammalianMutationS472APromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#2
Plasmid#70094PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#1
Plasmid#70093PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-RUNX2-IDR-mCherry-CRY2-NLS
Plasmid#145267PurposeMammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLSDepositorInsertRUNX2wt-IDR (RUNX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/FKBP12-pBirA(284-C)-HA
Plasmid#154452Purposeexpresses FKBP12-pBirA(284-C)-HA/biochemical assayDepositorInsertFKBP12-pBirA(284-C)-HA (FKBP12 Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only