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Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…TagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mc2 neo EF1a
Plasmid#206220PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-C1
Plasmid#54801PurposeLocalization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsmCherry-miniSOGExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAL119
Plasmid#21909DepositorInserthCMV promoter expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3E-ML1N*2
Plasmid#67678PurposeMultisite gateway entry clone for adding C-terminal fusions of ML1N*2DepositorInsertML1N*2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD103-ARG1
Plasmid#106475PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimuriumDepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLuxAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-Venus
Plasmid#87613PurposeActin-targeted Venus (YFP).DepositorInsertLifeAct-Venus
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZF39: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104118PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCNA_PCNA
Plasmid#110022PurposeProtein expression and purification of PCNA_PCNADepositorInsertPCNA_PCNA (PCNA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_WT-talin1 (1-2541)
Plasmid#166112PurposeFor expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertWT-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-sapphireAKAR
Plasmid#118464Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertsapphireAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo1
Plasmid#157805PurposeExpression of synthetic protein composed of eGFP and BRD4 bromodomain #1DepositorInserteGFP-bromo1
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only